let me map this out for you. I have a cup of water, like an actually big gulp cup that I have added two drops of API to it and want to know how long it will take before I can add it to my tank so I can refill it and condition that water. Also I have a 1 gal jug that I have put damn near 15 drops in (just to be safe) how long before I can add that to the tank? I drained 2.5 gals from my tank and want to make sure I can fill it all up today. All the water has settled to room temp so it's a bit colder than the tank water left right now. I am mostly concerned with adding the water in the big gulp cup first hoping since it's smaller it should take less time to condition. That way my fish isn't stuck in this tiny amount of water for too long.