less than 2 inches in length can be euthanized by exposing them to freezing cold water. The fish is put into a small container along with some aquarium water at the normal temperature. This container is then placed into a much larger container filled with crushed ice. This will rapidly chill the water in the smaller container, eventually rendering the fish unconscious. When death is verified, the fish can be removed. Decapitation
While too grisly for most aquarists, stunning a fish, decapitating it and then pithing it (physically destroying the brain with a metal rod) is a humane way to euthanize a fish. Because fish can remain conscious for some time after decapitation, the pithing step is essential. If you don’t know how to pith a fish, then don’t use this method.
Also known as eugenol, clove oil is a sedative at low doses, but at higher doses it has been recommended by some researchers as an inexpensive way to euthanize fish, particularly small fish. In a container, mix aquarium water with clove oil and mix. When exposed to high concentrations of clove oil, fish quickly lose consciousness and stop breathing, both of which reduce pain. Hypoxia eventually causes death, and once verified, the fish can be removed from the water and clove oil mixture. The required dose for euthanasia is 400 mg/l or more.
Clove oil contains some substances believed to be carcinogenic and should be handled with care.
Pressurized carbon dioxide vigorously bubbled through water for at least 30 seconds will quickly displace dissolved oxygen. A fish placed in such a container will quickly become unconscious and soon die, assuming the fish cannot breathe atmospheric air (many freshwater fish can: check before using this method). Once death is verified, the fish can be removed.
Note that this is not the same thing as methods based on Alka-Seltzer tablets or carbonated drinking water, neither of which will produce the environmental conditions described. Such methods have not been proven to be humane, are not used by vets and cannot be recommended here.
By administering an overdose of an anesthetic, vets can ensure that large fish are humanely euthanized. This is the method recommended for use with large fish, such as koi, oscars and saltwater angelfish. Because large fish will be stressed by being caught and transported to a veterinarian, the vet may need to visit the fishkeeper’s home and euthanize the fish there.
A variety of anesthetics have been used for this purpose, including 2-phenoxyethanol (bath, 0.3 to 0.4 mg/liter); benzocaine hydrochloride (bath, at least 250 mg/liter); sodium pentobarbital (injection, 60 to 100 mg/kg body weight); tricaine methanesulphonate, also known as MS222 (bath, 300 mg/liter). Because tricaine methanesulphonate is acidic, it will need to be used alongside an appropriate pH buffer. You need a pH buffer if you have fish from a non-acidic aquarium. Taking a marine fish from an aquarium at pH 8.5 and dumping it into a bath containing MS222 solution at around pH 6.5 will be intensely stressful. So if using this chemical, buffer the water to the correct pH before adding the fish.
It may take more than 30 minutes for death to occur, and it is recommended that fish be left in anesthetic baths for at least 2 hours to be sure. Death will need to be verified before the fish is removed.
Disposing of Dead Fish
Dead fish should not be flushed. The body can be burned, disposed of in the household trash or buried in the garden away from bodies of water. If placed in the trash, wrap the body in paper or plastic film to prevent it from being consumed by rats, cats and other garbage feeders. If buried in the garden, place the body deep enough underground that scavengers will not unearth it.