Well, yesterday i decided to set up my 37 gal. tank, i did everything i should have. I rinsed the gravel and tank with water along with the plants and accesories. After i put everything back into the tank i added water straight from the garden hose and decholornaited it. I let the water stand until it got to about 80*F 26*C and then added my guppies, dwarf frog and ghost shrimp. Today when i woke up i found all of my male guppies dead and 1 female. Only 1 preganant female is left and is at the top of the tank gasping for air. I also have 2 guppy fry that are still alive too. I dont think it was ammonia poisioning because the fry survived, and the frog and shrimp are doing well.