The amount of nitrifying bacteria mainly depends on one thing, other than the amount of oxygen, light, etc. mostly food. As in ammonia. The amount of nitrosifyers can very greatly depending on the amount of food, in this case ammonia present. Nitrosifyers, such as nitrosococcus, And nitrosomonas. (there are more types of nitrosifyers but these are the only in an FW system) will convert toxic substances such as ammonia into equally toxic ones, in this particular situation, it would be nitrites, nitrites then get converted into less harmful substances by nitrifying bacteria. By nItrobacter (there are more types of nitrosifyers but these are the only in an FW system) the result of this would be nitrates. So the amount of nitrates you have can represent the amount of ammonia being produced if you don't have any plants in there or any other way to remove nitrates. You would want to achieve media from an over stocked tank or from a very established fully stocked tank. And take about 1/3 or the media. It should cycle in a small amount of time. Around 1-2 weeks